Lulzbot 3d Bio Printer FRESH Certified


The LulzBot Bio is a FRESH certified bioprinter ready for use with bioinks, unmodified collagen, and other soft materials for pharmaceutical/cosmetic testing, tissue engineering, and regenerative medicine.

The LulzBot Bio combines versatility with exceptional print quality in a compact and easy-to-use bioprinter. Certified for FRESH printing, the Bio includes everything you need to get started printing bioinks, unmodified collagen, and other soft materials. With an Open Source design and less than an hour of out-of-the-box setup time, the Bio’s capabilities can grow as fast as your innovation.

Bioprinting is revolutionizing pharmaceutical development, cosmetic testing, tissue engineering, and regenerative medicine. 3D bioprinted tissues can be used to mimic in vivo conditions, enabling organizations to better predict efficacy and toxicology of potential drugs early on in the drug discovery process.

What have they printed with a LulzBot Bio?

  • Functional tissues with contractility (includes heart tissues)
  • Collagen heart valve that can support pulsatile flow
    • Have not tested in animals yet but have simulated blood flow
  • Beating ventricle made of human heart cells
  • Multi-scale vasculature (from 5mm down to 100 μm)
    • Was able to support cells living around that vessel network
  • Artery trees


  • Sodium Alginate – Rapid prototyping material derived from brown seaweed.
    • Translucent therefore requires dye to be seen when suspended in water/alcohol.
    • LifeSupport must be mixed with calcium chloride 0.1% solution to enable crosslinking.
  • Collagen – Highly important protein in the body.
    • The LulzBot Bio is ready to print unmodified collagen. This is very important as we are closer to mimicking human physiology.
    • Pure collagen can be printed down to 20 μm.
    • Can be crosslinked with a PH change via addition of acetic acid (main component of vinegar apart from water).
  • Cells –
    • Usually these are stem cells.
    • Can only be printed down to 100 μm needle. Smaller needles will impact cell viability.
    • Cells need to touch each other to maintain cell viability.
    • After printing they must be placed in cell culture incubator at 37°c
  • More to come: Fibrin, hyaluronic acid, termosets


Available on backorder


Add-ons total:


SKU: 13847 Category:



Syringe Pump Extruder

  • The syringe pump extruder design allows for precise control of material extrusion, enabling printing of high-detail objects in a wide range of materials with varying viscosities.
    • Note: The syringe pump extruder uses Hamilton Gas Tight 1000 Series Syringes (81420, 81520, and 81620) with X-Style Plunger and Luer Lock termination. The Bio will come with one Hamilton 2.5 mL Gastight Syringe Model 1002 TLL.

Ease to Use Interface

  • The Bio features a 4.5” glove-friendly touch screen and SD card slot for easy operation without the need to be tethered to a computer.

Material Options

  • The LulzBot Bio’s open materials system includes ready-to-print profiles for alginate and unmodified collagen. With a configurable syringe pump system and extrusion settings, the Bio is easily adjustable for new materials and processes.

Designed For The Lab

  • The small footprint and large build volume easily fits within laminar flow cabinets or on lab countertops. The rugged design can easily be sterilized for printing cells using isypropyl alcohol and UV lights.

Bed Heating

  • The LulzBot Bio’s variable temperature heated bed allows full control of environmental conditions during printing, ensuring the integrity of the print from start to removal.


Print Technology Fluid Deposition Fabrication

Print Head Syringe Pump Extruder

Maximum Print Area 160mm x 160mm x 89mm1 (6.30in x 6.30in x 3.5in1)

Minimum Layer Resolution 50 µm2

Minimum Positive Feature Size 80 µm3

Calculated XYZ Positional Resolution 10, 10, 5 µm

Recommended Operating Temperature From 5°C to 40°C (41°F to 104°F)

Operating Footprint 45.7cm x 40.6cm (18in x 16in)

Connectivity USB Serial and SD Card

Print Surface Heated Borosilicate Glass

Maximum Print Surface Temperature Up to 120°C (248°F)

Print Surface Heat Up Time 23°C to 60°C (73°F to 140°F) in 97 seconds

Interface Glove-Friendly 4.5” Touch-Screen

Materials Open materials system including ready-to-print profiles for alginate and collagen.


Operating System Compatibility GNU/Linux, Mac, Windows

Recommended Software Cura LulzBot Edition (Latest Version)

Firmware Marlin

Supported File Types .stl, .obj, .g, .gcode, .x3d, .3mf


Power Requirements 100VAC-240VAC, 50Hz-60Hz, 3.2A max

Output 24VDC

Power Supply IEC cord, C13 to region-specific connector

Safety and Compliance

Certifications UL (Pending), FCC, CE, WEEE, CSA, RCM, OSHWA

Warranty Includes one-year warranty and access to technical support seven days a week. Optional one, two, or three-year extended warranty and support terms are available.

Country of Origin Made in USA from domestic and imported components.

What’s Included** LulzBot Bio

Power Cord

USB Cord

SD Card

Quick Start Guide

50g Sodium Alginate

LifeSupport™ support material

1g Alcian Blue 8GX – Water soluble cationic dye

Calcium Chloride

Hamilton 2.5 mL Gastight Syringe Model 1002 TLL

Box of needles (50pk) – 25 gauge

Box of needles (50pk) – 30 gauge

2 Syringe thumb screws

35mm Petri dish

Glass 50mL Crystallizing Dish

Vacuum grease

Allen wrench set

Long nose pliers




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